Journal: PLoS ONE

Article Title: In Vivo Serial MR Imaging of Magnetically Labeled Endothelial Progenitor Cells Homing to the Endothelium Injured Artery in Mice

doi: 10.1371/journal.pone.0020790

Figure Lengend Snippet: 7 days after in vitro differentiation, spleen-derived EPCs showed expression of CD31 (B), CD34 (C) and vWF (D) with the cytoplasm stained in brown. Photo A is the control. Hematoxylin-stained nuclei were shown in blue. (Magnification ×400).

Article Snippet: After 7 days in culture, spleen-derived EPCs in 4 wells of a 24-well culture plate were extensively washed, and fixed in ice-cold 4% paraformaldehyde for 20 min. Immunocytochemistry was performed using antibodies against CD31, CD34 (Wuhan Boster Biological Technology, LTD. Wuhan, China) and von Willebrand factor (vWF) ( Zhongshan Goldenbridge Biotechnology Co., LTD. Beijing, China) according to standard protocols.

Techniques: In Vitro, Derivative Assay, Expressing, Staining

Journal: Cell communication and signaling : CCS

Article Title: O-GlcNAcylated FTO promotes m6A modification of SOX4 to enhance MDS/AML cell proliferation.

doi: 10.1186/s12964-025-02058-6

Figure Lengend Snippet: Fig. 2 O‑GlcNAcylation weakens FTO expression. (A) Volcano diagram representing FTO expression after OSMI-1 treatment in KG1a cells; p-val ue < 0.05,|Fold change| > 1.5. (B) The correlation between O-GlcNAc levels and the survival of AML patients was explored using upper quartile analysis through the Kaplan-Meier Plotter. (C) The association between O-GlcNAc and FTO in a cohort of 14 MDS/AML patients by ELISA. (D) Western blot detec tion of FTO level in cKit + cells of the bone marrow from MLL-AF9-induced leukemia mice. (E) FTO level in primary CD34 + cells from MDS/AML patients after 20 µM DMSO and 20 µg/mL OSMI-1 treatment. (F-G) FTO level in KG1a and SKM1 cells after TMG and OSMI-1 treatment

Article Snippet: CD34+ cells were treated with 20 μM DMSO and 20 μg/mL OSMI-1 (MCE, NJ, USA) for 48 h, respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Journal of analytical atomic spectrometry

Article Title: Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

doi: 10.1039/b921770c

Figure Lengend Snippet: Fig. 7 Examples of bivariate plots of mass cytometric results for (A) free AA120-Eu beads and (B) KG1a free cells stained with CD34-169Tm and Ir-interchelator.

Article Snippet: Human monocyte cell line KG1a, a model human acute myelogenous leukemia cell line, with high CD34 antigen expression (approximately 100 000 copies per cell), as well as This journal is a The Royal Society of Chemistry 2010 U937 human leukemic monocyte lymphoma cell line (that does not express CD34), were obtained from the American Type Culture Collection (Manassas, VA).

Techniques: Staining

Journal: Journal of analytical atomic spectrometry

Article Title: Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.

doi: 10.1039/b921770c

Figure Lengend Snippet: Fig. 9 Comparison between the 193Ir (from DNA intercalator) and 169Tm (from CD34-169Tm) averages of integrated ion intensities over the tran- sient signals for KG1a cells gated alone (x-axis) and in a mixture with AA120-Eu beads (y-axis).

Article Snippet: Human monocyte cell line KG1a, a model human acute myelogenous leukemia cell line, with high CD34 antigen expression (approximately 100 000 copies per cell), as well as This journal is a The Royal Society of Chemistry 2010 U937 human leukemic monocyte lymphoma cell line (that does not express CD34), were obtained from the American Type Culture Collection (Manassas, VA).

Techniques: Comparison

Journal: Heliyon

Article Title: Wharton's jelly derived mesenchymal stem cells differentiate into oocyte like cells in vitro by follicular fluid and cumulus cells conditioned medium

doi: 10.1016/j.heliyon.2020.e04992

Figure Lengend Snippet: Flow cytometry histograms of WJ-MSCs and BM-MSCs . WJ-cells were positive for mesenchymal markers CD73 (A), CD90 (B), CD 105 (C) and negative for hematopoietic markers CD34/CD45 (D). This data compared with Positive expression of mesenchymal markers CD90 (F), CD73 (G), CD 105 (H) in BM-MSCs. Flow cytometry analysis of WJ-MSCs were done on three independent experiments and their averages were indicated in diagram (E).

Article Snippet: Then, the cells were incubated for 30 min at 4 °C with anti-human antibodies against CD73-PE (2:100 μl, BD bioscience, 550257, USA), CD105-PE (2:100 μl, BD bioscience, 560839, USA), CD90-FITC (2:100 μl, BD bioscience, 555595, USA) and human leucocyte antigen CD45/CD34-FITC/PE (2:100 μl, BD Biosciences, 341071, USA).

Techniques: Flow Cytometry, Expressing